Abstract
Two alternative specific and very sensitive methods for determination of vinyl epoxide synthetase activity in liver microsomes are described.
Trichloroethylene, which is used as a substrate, is converted into trichloro-ethylene oxide by a hepatic epoxide synthetase. Chloral hydrate, the final rearrangement product of trichloroethylene oxide, is evaluated by electron capture gas chromatography, either after derivatization with pentafluorophenyl-hydrazine or after its conversion into chloroform under alcaline conditions. The kinetic parameters of the epoxidative reaction have been determined on rat hepatic microsomal suspensions.
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Malvoisin, E., Rollmann, B., Roberfroid, M., Mercier, M. (1978). A Gas Chromatographic Method for the Evaluation of the Vinyl Epoxidase Activity. In: Leonard, B.J. (eds) Toxicological Aspects of Food Safety. Archives of Toxicology, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66896-8_60
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DOI: https://doi.org/10.1007/978-3-642-66896-8_60
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-08646-8
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