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Correlation of the properties of several lignocellulosic substrates to the crop performance of the shiitake mushroom Lentinula edodes

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Abstract

Two selected Lentinula edodes strains (S4080 and SIEF0231) were cultivated on oak-wood sawdust (OS), wheat straw (WS) and corn-cobs (CC) substrates in order to examine the influence of those residues on mycelium growth and on basidiomata production. For both strains, mycelial growth measurements conducted in ‘race tubes’ demonstrated faster colonization of OS and WS media. Lag-phase and complete colonization periods were correlated to mycelium extension rates in the three substrates tested. Similar patterns of pH and electrical conductivity (Ec) changes were detected in all media and for all strains tested; the pH decreased steadily throughout the colonization process to reach values of 4.49–5.06; Ec increased by the end of mycelium colonization, and it presented the highest and lowest values in the WS and OS media respectively. In addition, a negative correlation was established between final salt content of the substrates and mycelium extension rates. Subjecting fully colonized substrates to a cold-shock treatment resulted in fruiting 58–65 days after inoculation in tubes; WS and CC promoted earlier sporophore initiation than OS. Monitoring CO2 emissions by strain SIEF0231 in pilot-scale cultivation on synthetic blocks, revealed higher respiration rates from OS and CC than from WS, which were further correlated with substrate colonization rates. Among residues colonized by the same strain, WS appeared to promote earliness and crop productivity (BE 54.17%) by presenting shorter cropping periods and equal yield distribution among flushes, while on OS and CC maximum yields were obtained within the first two flushes. Moreover, heavier basidiomata were produced by WS and OS substrates.

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References

  • Badham, E.R. 1989 Influence of water potential on growth of shiitake mycelium. Mycologia 81, 464–468.

    Google Scholar 

  • Bis'ko, N.A. & Bilay, V.T. 1996 Some physiological aspects of the cultivation of Lentinula edodes (Berk.) Sing. In Mushroom Biology and Mushroom Products, ed. Royse, D.J. pp. 381–386. Pensylvania State University. ISBN 1–883956–01–3.

  • Boyle, C.D. 1998 Nutritional factors limiting the growth of Lentinula edodes and other white-rot fungi in wood. Soil Biology and Biochemistry 30, 817–823.

    Google Scholar 

  • Buswell, J.A. & Chang, S.T. 1993 Edible mushrooms: attributes and applications. In Genetics and Breeding of Edible Mushrooms, eds. Chang, S.T., Buswell, J.A. & Miles, P.G. pp. 297–324. Philadelphia: Gordon and Breach Scientific Publishers. ISBN 2–88124561–7.

    Google Scholar 

  • Campbell, A.C. & Racjan, M. 1999 The commercial exploitation of the white rot fungus Lentinula edodes (shiitake). International Biodeterioration and Biodegradation 43, 101–107.

    Google Scholar 

  • Chen, A.W., Arrold, N. & Stamets, P. 2000 Shiitake cultivation systems. Mushroom Science 15, 771–778.

    Google Scholar 

  • Chihara, G. 1993 Medicinal aspects of lentinan isolated from Lentinus edodes (Berk.) Sing. In Mushroom Biology and Mushroom Products, eds. Chang, S.T., Buswell, J.A. & Chiu, S.W. pp. 261–266. Hong Kong: The Chinese University Press. ISBN 962201610–3.

    Google Scholar 

  • Donoghue, J.D. & Denison, W.C. 1995 Shiitake cultivation: gas phase during incubation influences productivity. Mycologia 87, 239–244.

    Google Scholar 

  • Eriksson, K-E.L., Blanchette, R.A. & Ander, P. 1990 Microbial and Enzymatic Degradation of Wood and Wood Components. New York: Springer-Verlag. ISBN 0–38751600-X.

    Google Scholar 

  • Freer, S. & Detroy, R. 1982 Biological delignification of 14C-labeled lignocelluloses by basidiomycetes: degradation and solubilization of lignin and cellulose components. Mycologia 74, 943–951.

    Google Scholar 

  • Kaal, E.E., Field, J.A. & Joyce, T.W. 1995 Increasing lignolytic enzyme activities in several white-rot basidiomycetes by nitrogen-sufficient media. Bioresource Technology 53, 133–139.

    Google Scholar 

  • Kalberer, P.P. 1995 An investigation of the incubation phase of a shiitake (Lentinus edodes) culture. Mushroom Science 14, 375–383.

    Google Scholar 

  • Kalberer, P.P. 2000 Influence of urea and ammonium chloride on crop yield and fruit body size of shiitake (Lentinula edodes). Mushroom Science 15, 361–366.

    Google Scholar 

  • Kirchhoff, B. & Lelley, J. 1991 Investigations of Shiitake (Lentinus edodes (Berk.) Sing.) bag-log cultivation to increase the yield in Germany. Mushroom Science 13, 509–516.

    Google Scholar 

  • Leatham, G.F. 1985 Extracellular enzymes produced by the cultivated mushroom Lentinus edodes during degradation of a lignocellulosic medium. Applied and Environmental Microbiology 50, 859–867.

    Google Scholar 

  • Leatham, G.F. & Stahmann, M.A. 1987 Effect of light and aeration on fruiting of Lentinula edodes. Transactions of the British Mycological Society 88, 9–20.

    Google Scholar 

  • Levanon, D., Rothschild, N., Danai, O. & Masaphy, S. 1993 Bulk treatment of substrate for the cultivation of Shiitake mushrooms (Lentinula edodes) on straw. Bioresource Technology 45, 63–64.

    Google Scholar 

  • Mizuno, T. 1995 Shiitake, Lentinus edodes: functional properties for medicinal and food purposes. Food Reviews International 11, 111–128.

    Google Scholar 

  • Moyson, E. & Verachtert, H. 1991 Growth of high fungi on wheat straw and their impact on the digestibility of the substrate. Applied Microbiology and Biotechnology 36, 421–424.

    Google Scholar 

  • Pacumbaba, R.P. & Pacumbaba, R.O. Jr. 1999 Shiitake mushroom growth on the formulated culture media, production of spawn and basidiocarps in the laboratory. HortTechnology 9, 85–90.

    Google Scholar 

  • Philippoussis, A., Diamantopoulou, P., Zervakis, G. & Euthimiadou, H. 2001b The composition and the porosity of lignocellulosic substrates influence mycelium growth and respiration rates of Lentinus edodes (Berk.) Sing. International Journal of Medicinal Mushrooms 3, 198.

    Google Scholar 

  • Philippoussis, A., Diamantopoulou, P. & Zervakis, G. 2002 Monitoring of mycelial growth and fructification on Lentinula edodes on several lignocellulosic residues. In Mushroom Biology and Mushroom Products. eds Sánchez, S.E., Hverta, G. & Montiel, E. pp. 279–287. Universidad Autónoma del Estado de Mocelos. ISBN 968–878–105–3.

  • Philippoussis, A. & Zervakis, G. 2000 Cultivation of edible mushrooms in Greece: presentation of the current status and analysis of future trends. Mushroom Science 15, 843–848.

    Google Scholar 

  • Philippoussis, A., Zervakis, G. & Diamantopoulou, P. 2001a Bioconversion of lignocellulosic wastes through the cultivation of the edible mushrooms Agrocybe aegerita, Volvariella volvacea and Pleurotus spp. World Journal of Microbiology and Biotechnology 17, 191–200.

    Google Scholar 

  • Philippoussis, A., Zervakis, G., Diamantopoulou, P. & Ioannidou, S. 2000 Potential for the cultivation of exotic mushroom species by exploitation of Mediterranean agricultural wastes. Mushroom Science 15, 523–530.

    Google Scholar 

  • Pire, D.G., Wright, J.E. & Alberto, E. 2001 Cultivation of shiitake using sawdust from widely available local woods in Argentina. Micologia Aplicada International 13, 87–91.

    Google Scholar 

  • Przybylowicz, P. & Donoghue, J. 1990 Shiitake Growers Handbook, The Art and science of Mushroom cultivation. Kendall/Hunt Publishing Company. ISBN 0–84034962–9.

  • Rayner, A.D.M. & Boddy, L. 1988 Fungal Decomposition in Wood: its Biology and Ecology. New York: John Wiley. ISBN 0–471–10310–1.

    Google Scholar 

  • Royse, D.J. 1995 Specialty mushrooms. Mushroom News 43, 4–19.

    Google Scholar 

  • Royse, D.J. & Bahler, C.C. 1986 Effects of genotype, spawn run time and substrate formulation on biological efficiency of shiitake. Applied and Environmental Microbiology 52, 1425–1427.

    Google Scholar 

  • Royse, D.J., Bahler, B.D. & Bahler, C.C. 1990 Enhanced yield of shiitake by saccharide amendment of the synthetic substrate. Applied and Environmental Microbiology 56, 479–482.

    Google Scholar 

  • Royse, D.J. & Sanchez-Vazquez, J.E. 2001 Influence of substrate wood-chip particle size on shiitake (Lentinula edodes) yield. Bioresource Technology 76, 229–233.

    PubMed  Google Scholar 

  • Sabota, C. 1996 Strain of shiitake mushroom [Lentinula edodes (Berk.) Pegler] and wood species affect the yield of shiitake mushrooms. HortScience 6, 388–392.

    Google Scholar 

  • Salmones, D., Mata, G., Ramos, L.M. & Waliszewski, K.N. 1999 Cultivation of shiitale mushroom, Lentinula edodes, in several lignocellulosic materials originating from subtropics. Agronomie 19, 13–19.

    Google Scholar 

  • Smith, C. 1997 Commercial production of shiitake substrates. Mushroom World 3, 4–12.

    Google Scholar 

  • Stamets, P. 1993 Growing Gourmet and Medicinal Mushrooms. Berkley: Ten Speed Press. ISBN 0–89815–608–4.

    Google Scholar 

  • Tan, Y.H. & Moore, D. 1992 Convenient and effective methods for in vitro cultivation of mycelium and fruiting bodies of Lentinus edodes. Mycological Research 96, 1077–1084.

    Google Scholar 

  • Van Soest, P.J. & Wine, R.H. 1968 Determination of lignin and cellulose in acid detergent fiber with permanganate. AOAC Journal 51, 780–785.

    Google Scholar 

  • Wasser, S. & Weis, A. 1999 Medicinal properties of substances occurring in higher basidiomycetes mushrooms: current perspectives (Review). International Journal of Medicinal Mushrooms 1, 31–62.

    Google Scholar 

  • Zervakis, G. & Philippoussis, A. 2000 Management of agro-industrial wastes through the cultivation of edible mushrooms. In Proceedings of IV European Waste Forum 'Innovation in waste management', pp. 87–90. Milan: C.I.P.A. ISSN 1121–8215.

    Google Scholar 

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Philippoussis, A., Diamantopoulou, P. & Zervakis, G. Correlation of the properties of several lignocellulosic substrates to the crop performance of the shiitake mushroom Lentinula edodes . World Journal of Microbiology and Biotechnology 19, 551–557 (2003). https://doi.org/10.1023/A:1025100731410

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